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resource source identifier antibodies anti ythdf1 proteintech  (Proteintech)


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    Proteintech resource source identifier antibodies anti ythdf1 proteintech
    Figure 5. The <t>m6A/YTHDF1/APC</t> pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
    Resource Source Identifier Antibodies Anti Ythdf1 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+antibodies+anti+ythdf1+proteintech/YTHDF1+Antibody/pm40402742-218-2-7
    Average 96 stars, based on 400 article reviews
    resource source identifier antibodies anti ythdf1 proteintech - by Bioz Stars, 2026-08
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    1) Product Images from "m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development."

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.115727

    Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
    Figure Legend Snippet: Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Techniques Used: Cell Culture, In Vivo, Expressing

    Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation
    Figure Legend Snippet: Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Techniques Used:



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    resource source identifier antibodies anti ythdf1 proteintech  (Proteintech)
    96
    Proteintech resource source identifier antibodies anti ythdf1 proteintech
    Figure 5. The <t>m6A/YTHDF1/APC</t> pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
    Resource Source Identifier Antibodies Anti Ythdf1 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+antibodies+anti+ythdf1+proteintech/YTHDF1+Antibody/pm40402742-218-2-7
    Average 96 stars, based on 1 article reviews
    resource source identifier antibodies anti ythdf1 proteintech - by Bioz Stars, 2026-08
    96/100 stars
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    resource source identifier antibodies rabbit polyclonal anti ythdf1 proteintech  (Proteintech)
    96
    Proteintech resource source identifier antibodies rabbit polyclonal anti ythdf1 proteintech
    Figure 1. YTHDF2 is complexed with UPF1 and PNRC2 (A) Immunoprecipitation (IP) of endogenous YTHDF2. The extracts of HEK293T cells either treated or not treated with RNase A were subjected to IPs with an anti (a)-YTHDF2 antibody or rabbit immunoglobulin G (rIgG) as a control. The protein samples before and after the IPs were analyzed using western blotting. (B) IPs of <t>YTHDF1,</t> YTHDF2, and YTHDF3. The extracts of HEK293T cells either untreated (left) or treated (right) with RNase A were subjected to IPs.
    Resource Source Identifier Antibodies Rabbit Polyclonal Anti Ythdf1 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+antibodies+anti+ythdf1+proteintech/YTHDF1+Antibody/pm35613594-231-2-9
    Average 96 stars, based on 1 article reviews
    resource source identifier antibodies rabbit polyclonal anti ythdf1 proteintech - by Bioz Stars, 2026-08
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    Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques: Cell Culture, In Vivo, Expressing

    Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques:

    Figure 1. YTHDF2 is complexed with UPF1 and PNRC2 (A) Immunoprecipitation (IP) of endogenous YTHDF2. The extracts of HEK293T cells either treated or not treated with RNase A were subjected to IPs with an anti (a)-YTHDF2 antibody or rabbit immunoglobulin G (rIgG) as a control. The protein samples before and after the IPs were analyzed using western blotting. (B) IPs of YTHDF1, YTHDF2, and YTHDF3. The extracts of HEK293T cells either untreated (left) or treated (right) with RNase A were subjected to IPs.

    Journal: Cell reports

    Article Title: UPF1 promotes rapid degradation of m 6 A-containing RNAs.

    doi: 10.1016/j.celrep.2022.110861

    Figure Lengend Snippet: Figure 1. YTHDF2 is complexed with UPF1 and PNRC2 (A) Immunoprecipitation (IP) of endogenous YTHDF2. The extracts of HEK293T cells either treated or not treated with RNase A were subjected to IPs with an anti (a)-YTHDF2 antibody or rabbit immunoglobulin G (rIgG) as a control. The protein samples before and after the IPs were analyzed using western blotting. (B) IPs of YTHDF1, YTHDF2, and YTHDF3. The extracts of HEK293T cells either untreated (left) or treated (right) with RNase A were subjected to IPs.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-YTHDF1 Proteintech Cat#17479-1-AP; RRID: AB_2217473 Rabbit polyclonal anti-YTHDF2 Proteintech Cat#24744-1-AP; RRID: AB_2687435 Mouse monoclonal anti-YTHDF3 Santa Cruz Biotech Cat#sc-377119; RRID: AB_2687436 Rabbit polyclonal anti-HRSP12 Thermo Scientific Cat#PA5-31352; RRID: AB_2548826 Rabbit polyclonal anti-POP1 Proteintech Cat#12029-1-AP; RRID: AB_2166477 Rabbit monoclonal anti-CNOT1 Proteintech Cat#14276-1-AP; RRID: AB_10888627 Rabbit polyclonal anti-SMG1 Bethyl Laboratories Cat#A300-394A; RRID: AB_386105 Rabbit polyclonal anti-SMG5 Abcam Cat#ab33033; RRID: AB_882612 Rabbit polyclonal anti-SMG6 Laboratory of Drs. Akio Yamashita and Shigeo Ohno N/A Rabbit polyclonal anti-SMG7 Bethyl Laboratories Cat#A302-170A; RRID: AB_1659842 Rabbit polyclonal anti-DCP1A Sigma-Aldrich Cat#D5694; RRID: AB_1840796 Mouse monoclonal anti-FLAG Sigma-Aldrich Cat#A8592; RRID: AB_439702 Rabbit polyclonal anti-GST Bethyl Laboratories Cat#A190-122A; RRID: AB_67419 Rat monoclonal anti-HA Roche Cat#11 867 423 001 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat#A5441; RRID: AB_476744 Mouse monoclonal anti MYC Calbiochem Cat#OP10L; RRID: AB_2266865 Rabbit polyclonal anti-UPF1 Cho et al., 2012 N/A Rabbit polyclonal anti-UPF2 Cho et al., 2012 N/A Rabbit polyclonal anti-PNRC2 Cho et al., 2009 N/A Goat anti-mouse IgG, peroxidase conjugated Sigma-Aldrich Cat#AP124P; RRID: AB_90456 Goat anti-rabbit IgG, peroxidase conjugated Sigma-Aldrich Cat#AP132P; RRID: AB_90264 Chemicals, peptides, and recombinant proteins Cycloheximide Sigma-Aldrich Cat#01810 5,6-dichloro-1-b-D ribofuranosylbenzimidazole (DRB) Sigma-Aldrich Cat#D1916 Plasmocin Invivogen Cat#ant-mpt Critical commercial assays rProtein A agarose 4B for Immunoprecipitation Incospharm Cat#1106-3 rProtein G agarose 4B for Immunoprecipitation Incospharm Cat#1105-3 FLAG M2 affinity gel Sigma-Aldrich Cat#A2220 MycoAlert PLUS Mycoplasma detection kit Lonza Cat#LT07-701 Poly(A) Tail-Length Assay Kit Thermo Scientific Cat#764551KT Deposited data PAR-CLIP and RIP-seq Wang et al., 2014 SRP028325 PARE-seq Park et al., 2019 SRP155278 BRIC-seq Imamachi et al., 2017 DRA000591 Mendeley Data dataset This paper https://doi.org/10.17632/hmdttb83rd.1 Zenodo Data dataset This paper https://doi.org/10.5281/zenodo.6487620 (Continued on next page) Cell Reports 39, 110861, May 24, 2022 e1

    Techniques: Immunoprecipitation, Control, Western Blot

    Figure 5. The UPF1-mediated rapid degradation pathway targets mRNAs containing YTHDF2-binding sites at the transcriptome level (A) Cumulative distribution function (CDF) plots for relative changes in the half-life of mRNAs. CDF plots of nontargets (3,048), CLIP targets (2,740), and CLIPXRIP targets (1,159) were plotted as relative changes in the ratio of half-life after the downregulation of UPF1. (B) CDF plots of mRNAs with a YTHDF2 peak among CLIPXRIP targets. The mRNAs (612) with the YTHDF2 peak among CLIPXRIP targets (1,159) were cate- gorized into the following three groups depending on the position of the YTHDF2 peak: 50 UTR (20), CDS (325), and 30 UTR (267). CDFs were plotted as relative changes in half-life after the downregulation of UPF1. (C) Venn diagrams for the numbers of mRNAs belonging to groups Y–P (CLIPXRIP) and YXP. According to the reanalyzed data on groups Y (CLIPXRIP) and P (PARE-seq), cellular mRNAs were categorized into the following two groups: YXP targets (210 mRNAs, present in both groups Y and P) and Y-P targets (983 mRNAs, present only in the Y group). (D) CDF plots of the relative change in the half-life of mRNAs. CDF plots for nontargets (3,048), YXP (203), and Y–P (956) were plotted as relative changes in half- life after the downregulation of UPF1. (E) CDF plots of the relative change in the half-life of mRNAs harboring a binding site for YTHDF1, -2, or -3 after the downregulation of UPF1. (F) CDF plots of the relative change in the half-life of mRNAs harboring a binding site only for YTHDF1, -2, or -3 after the downregulation of UPF1. p values were computed using the Kruskal-Wallis test and post hoc Dunn’s test with the Bonferroni correction.

    Journal: Cell reports

    Article Title: UPF1 promotes rapid degradation of m 6 A-containing RNAs.

    doi: 10.1016/j.celrep.2022.110861

    Figure Lengend Snippet: Figure 5. The UPF1-mediated rapid degradation pathway targets mRNAs containing YTHDF2-binding sites at the transcriptome level (A) Cumulative distribution function (CDF) plots for relative changes in the half-life of mRNAs. CDF plots of nontargets (3,048), CLIP targets (2,740), and CLIPXRIP targets (1,159) were plotted as relative changes in the ratio of half-life after the downregulation of UPF1. (B) CDF plots of mRNAs with a YTHDF2 peak among CLIPXRIP targets. The mRNAs (612) with the YTHDF2 peak among CLIPXRIP targets (1,159) were cate- gorized into the following three groups depending on the position of the YTHDF2 peak: 50 UTR (20), CDS (325), and 30 UTR (267). CDFs were plotted as relative changes in half-life after the downregulation of UPF1. (C) Venn diagrams for the numbers of mRNAs belonging to groups Y–P (CLIPXRIP) and YXP. According to the reanalyzed data on groups Y (CLIPXRIP) and P (PARE-seq), cellular mRNAs were categorized into the following two groups: YXP targets (210 mRNAs, present in both groups Y and P) and Y-P targets (983 mRNAs, present only in the Y group). (D) CDF plots of the relative change in the half-life of mRNAs. CDF plots for nontargets (3,048), YXP (203), and Y–P (956) were plotted as relative changes in half- life after the downregulation of UPF1. (E) CDF plots of the relative change in the half-life of mRNAs harboring a binding site for YTHDF1, -2, or -3 after the downregulation of UPF1. (F) CDF plots of the relative change in the half-life of mRNAs harboring a binding site only for YTHDF1, -2, or -3 after the downregulation of UPF1. p values were computed using the Kruskal-Wallis test and post hoc Dunn’s test with the Bonferroni correction.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-YTHDF1 Proteintech Cat#17479-1-AP; RRID: AB_2217473 Rabbit polyclonal anti-YTHDF2 Proteintech Cat#24744-1-AP; RRID: AB_2687435 Mouse monoclonal anti-YTHDF3 Santa Cruz Biotech Cat#sc-377119; RRID: AB_2687436 Rabbit polyclonal anti-HRSP12 Thermo Scientific Cat#PA5-31352; RRID: AB_2548826 Rabbit polyclonal anti-POP1 Proteintech Cat#12029-1-AP; RRID: AB_2166477 Rabbit monoclonal anti-CNOT1 Proteintech Cat#14276-1-AP; RRID: AB_10888627 Rabbit polyclonal anti-SMG1 Bethyl Laboratories Cat#A300-394A; RRID: AB_386105 Rabbit polyclonal anti-SMG5 Abcam Cat#ab33033; RRID: AB_882612 Rabbit polyclonal anti-SMG6 Laboratory of Drs. Akio Yamashita and Shigeo Ohno N/A Rabbit polyclonal anti-SMG7 Bethyl Laboratories Cat#A302-170A; RRID: AB_1659842 Rabbit polyclonal anti-DCP1A Sigma-Aldrich Cat#D5694; RRID: AB_1840796 Mouse monoclonal anti-FLAG Sigma-Aldrich Cat#A8592; RRID: AB_439702 Rabbit polyclonal anti-GST Bethyl Laboratories Cat#A190-122A; RRID: AB_67419 Rat monoclonal anti-HA Roche Cat#11 867 423 001 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat#A5441; RRID: AB_476744 Mouse monoclonal anti MYC Calbiochem Cat#OP10L; RRID: AB_2266865 Rabbit polyclonal anti-UPF1 Cho et al., 2012 N/A Rabbit polyclonal anti-UPF2 Cho et al., 2012 N/A Rabbit polyclonal anti-PNRC2 Cho et al., 2009 N/A Goat anti-mouse IgG, peroxidase conjugated Sigma-Aldrich Cat#AP124P; RRID: AB_90456 Goat anti-rabbit IgG, peroxidase conjugated Sigma-Aldrich Cat#AP132P; RRID: AB_90264 Chemicals, peptides, and recombinant proteins Cycloheximide Sigma-Aldrich Cat#01810 5,6-dichloro-1-b-D ribofuranosylbenzimidazole (DRB) Sigma-Aldrich Cat#D1916 Plasmocin Invivogen Cat#ant-mpt Critical commercial assays rProtein A agarose 4B for Immunoprecipitation Incospharm Cat#1106-3 rProtein G agarose 4B for Immunoprecipitation Incospharm Cat#1105-3 FLAG M2 affinity gel Sigma-Aldrich Cat#A2220 MycoAlert PLUS Mycoplasma detection kit Lonza Cat#LT07-701 Poly(A) Tail-Length Assay Kit Thermo Scientific Cat#764551KT Deposited data PAR-CLIP and RIP-seq Wang et al., 2014 SRP028325 PARE-seq Park et al., 2019 SRP155278 BRIC-seq Imamachi et al., 2017 DRA000591 Mendeley Data dataset This paper https://doi.org/10.17632/hmdttb83rd.1 Zenodo Data dataset This paper https://doi.org/10.5281/zenodo.6487620 (Continued on next page) Cell Reports 39, 110861, May 24, 2022 e1

    Techniques: Binding Assay